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Fig. S7 Loss of Rab11fip2 fails to rescue Ovo1 morphants. (A) RT-PCR for rab11fip2 to test morpholino (MO) efficacy (upper panel) using ef1a as a loading control (lower panel). Lanes: 1, control; 2, 0.75 ng Rab11fip2 MO (here referred to as fip2MO); 3, 1.5 ng fip2MO; 4, 3 ng fip2MO; 5, 3 ng Ovo1 MO; 6, 3 ng Ovo1 MO + 0.75 ng fip2MO; 7, 3 ng Ovo1 MO + 1.5 ng fip2MO; 8, 3 ng Ovo1 MO + 3 ng fip2MO. (B) Quantitation of embryos with ectopic NC cells in the dorsal midline (blue) in Ovo1 morphants and Rab11fip2 MO rescued embryos. (C-E) Live images of Gfp expression in NC cells (arrows) in controls (C), Ovo1 single morphants (D) and Ovo1MO, fip2MO double-morphant embryos (E); dorsal views. ot, otic vesicle.