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Fig. S5 Ncad overexpression does not rescue neural crest defects in Ovo1 morphants. (A) Quantitative PCR shows reduced ncad mRNA levels in Ovo1 morphants (*, P≤0.05). (B-E) In situ hybridization for sox10 in neural crest (NC). Dorsal views, anterior to the top. In contrast to controls (B), NC accumulates (arrows) in the dorsal midline of embryos injected with ncad mRNA (C), Ovo1 morphants (D) or a combination of ncad mRNA and Ovo1 MO (E). ot, otic vesicle.
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