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Fig. 6 Inhibition of intracellular trafficking rescues the Ovo1 morphant phenotype. (A-C) In situ hybridization for sox10 at 24 hpf. Dorsal views, anterior to the left. Percentages of embryos with NC cell aggregates at the dorsal midline (arrows) with or without BFA treatment are indicated at the bottom of each panel. (A) A severe example with >5 cells per aggregate over both the midbrain and hindbrain. (C) A less severe example with <5 cells per aggregate exclusively located over the hindbrain. (D-I) Ncad:Gfp injections. Ncad:Gfp localizes to the membranes (arrows) of untreated (D) and BFA-treated control cells (G). In Ovo1 morphant cells, Ncad:Gfp also accumulates in the cytoplasm (E,F; arrowheads), but is largely restored to the membrane in BFA-treated Ovo1 morphant cells (H,I). n, nucleus; ot, otic vesicle.