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Fig. S3 jag2, but not jag1a, is expressed in the pharyngeal arches and is regulated by Edn1. (A-C) Whole-mount in situ immunohistochemistry at 36 hpf. jag1a and jag1b show complementary, non-overlapping expression patterns, with jag1a expression in the central nervous system and jag1b expression in arch CNCCs and sensory and otic placodal cells. jag2 is expressed in the ventral brain and weakly in the pharyngeal arches. (D-F) Higher magnification views of jag2 expression at 36 hpf. In wild types, jag2 expression is restricted to dorsal CNCCs of the mandibular and hyoid arches and the mandibular arch artery (arrowhead). Compared with expression in edn1+/- siblings, jag2 expression in edn1-/- embryos is expanded into the ventral hyoid arch (arrow). Outlines of the eye and endodermal pouches are shown.