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Fig. 3 Localization of Pard3-GFP may be microtubule-independent. Sections of control (A, B, D) and nocodazole-treated (C) embryos at 6–7 som (A, C) and 12–13 som (B, D). (A–C) Embryos were mosaically injected at the 8–16 cell stage with pard3-GFP mRNA and immunolabeled with anti-GFP. (C) The nocodazole treatment was performed for 30 min at an early 6 som stage and the embryo was fixed immediately following treatment. (D) Embryo was injected with mGFP DNA and immunolabeled with anti-GFP. Arrowheads: apical localization of Pard3-GFP. Scale bar: 10 μm.
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Reprinted from Developmental Biology, 341(2), Hong, E., Jayachandran, P., and Brewster, R., The polarity protein Pard3 is required for centrosome positioning during neurulation, 335-345, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.