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Fig. 4 AGN192403 blocks cell proliferation. (A, B) Bromodeoxyuridine (BrdU) staining of frozen sections from 72 hpa fin regenerates. Fish were treated with 1% DMSO (A) or 25 μM AGN192403 (B) from 12 to 72 hpa. BrdU-labeled cells were counted if they were deep to the basement membrane (yellow lines) and within 1000 μm of the distal tip of the fin (white lines). (C) AGN192403 significantly decreased the number of BrdU-labeled cells compared to control treated fins (n = 5 fins per group, 20 sections per slide; data represent means ± SEM).
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