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Fig. S1 Morphology of RB neurons, CNS neurons and muscle is normal in mi310 mutants. (A-D) Anti-HuC and anti-acetylated tubulin labeled cell bodies and peripheral axons of RB neurons, respectively, in both wild-type (A,C) and mutant (B,D) embryos. (E,F) Reticulospinal neurons in hindbrain were visualized with 3A10 antibody. (G-L) Motoneurons and nicotinic acetylcholine receptors were labeled with znp-1 (G,H) and α-bungarotoxin (I,J), respectively, and their colocalizations represented neuromuscular junctions (K,L). (M-P) Fast muscle fibers (M,N) and slow muscle fibers (O,P) were visualized by DIC in both wild-type and mutant muscle.