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Fig. 6 Surface distribution of Scn1bb in RB neurons is diminished by knocking down GPI transamidase subunits. Dissociated RB neurons (GFP-positive) from 2 dpf embryos were labeled with anti-Scn1bb in the permeable (+TX-100) or impermeable (-TX-100) condition. (A-F) In control morpholino (MO)-injection, anti-Scn1bb labeled a whole RB cell in the presence of TX-100 (A-C), whereas it labeled the circumference of an RB cell in the absence of TX-100 (D-F). (G-I) Labeling with anti-Scn1bb with TX-100 displayed Scn1bb expression in an RB cell dissociated from a pigu morphant. (J-L) Scn1bb staining without TX-100 represented the impairment of Scn1bb surface distribution in a pigu morphant RB neuron. (M-O) Scn1bb labeling with TX-100 showed Scn1bb expression in a gpi8 morphant RB cell. (P-R) The surface expression of Scn1bb was not seen in an RB cell dissociated from a gpi8 morphant.