Fig. 3

Fig. 3 RPTPα knockdown abolished protein expression and both RPTPα and PTPε knockdowns were rescued by co-injection of RNA. (A) Zebrafish embryos were micro-injected at the 1 cell stage with ptpra-MO (0.1 ng/embryo or 0.4 ng/embryo) and grown to 52 hpf. Embryos were dechorionated, deyolked and lysed using sodium orthovanadate and protease inhibitors. Samples of non-injected control and MO injected embryos were loaded on SDS-PAGE in such a way that all samples contain equal amounts of protein. Immunoblots were analyzed for expression of RPTPα and actin as a loading control. (B–D) Zebrafish embryos were micro-injected with ptpra-MO (C) or ptpre-MO (D) with or without mRNA encoding mouse RPTPα (C) or PTPε (D). Embryos were grown to 2 dpf and phenotypes were assigned as indicated in panel B, where embryos scored as normal resemble non-injected control, embryos with overall normal looking phenotype and body plan but shortened body axis were labeled as intermediate, and embryos with severely disrupted morphology were labeled severe.