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Fig. S4 Reduced expression of dlx2 and gsc genes in edn1-knockdown embryos. Confocal images of control (A, D) and edn1-MO injected (B, C, E, F) embryos doubly transgenic for cldnb:gfp and atoh1a:dtomato at 52–54 hpf. Each embryo was fixed immediately after the observation and stained with the dlx2a (A′–C′) or gsc (D′–F′) RNA probes. The expression of both genes was severely reduced in morphant embryos, but the position of OP1 was not affected. Two morphant embryos are shown for each staining, which represent different severities of reduction in gene expression. Lines indicate positions of O2 and OP1. Scale bar: 50 μm.
Reprinted from Developmental Biology, 340(2), Wada, H., Ghysen, A., Satou, C., Higashijima, S.I., Kawakami, K., Hamaguchi, S., and Sakaizumi, M., Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish, 583-594, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.