ZFIN Image: Gates et al., 2010, Fig. 5

Image

Figure Caption

Fig. 5 Rest acts downstream of Smo in the Hh pathway. Lateral views of 24 hpf embryos injected with control MO (A, E) rest MO (B, F), dominant-negative PKA mRNA (dnPKA, C, G), or both (D, H) and stained for nkx2.2a. Embryos were placed in control media (A–D) or media containing 50 μM cyclopamine (E–H). Injection of dnPKA mRNA expands nkx2.2a expression (C). This expansion is augmented by co-injection with rest MO (D). CyA treatment partially attenuates the effects of dnPKA mRNA injection on nkx2.2a expression (G). dnPKA mRNA/rest mo injected embryos (F) are more resistant to CyA treatment then dnPKA treated embryos.

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Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.