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Fig. S7 Effect of MAPK inhibitors on the later Jun phosphorylation and regeneration. (A–D) Jun protein phosphorylation in the zebrafish finfold at 12 hpa (respective left side panels) and morphological regeneration at 3 dpa (respective right side panels) in the presence of DMSO (A; 0.05%), JNK inhibitor (B), p38 inhibitor (C) or MEK1/2 inhibitor (D). As was shown in Figs. 3A and B, the JNK inhibitor impaired the Jun phosphorylation and regeneration (red arrowheads), but no apparent effect was observed with inhibitors targeting the p38 and ERK, the latter being downstream of MEK1/2 (white arrowheads). The scale bar represents 50 μm in (A). (E) Quantification of regeneration in the inhibitor-treated larvae. Regeneration was evaluated by the finfold length posterior to the notochord. Statistical significance was tested by using Student′s t-test. *P < 0.001. N.S., not significant.
Reprinted from Developmental Biology, 340(2), Ishida, T., Nakajima, T., Kudo, A., and Kawakami, A., Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish, 468-479, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.