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Fig. S1 Onset of regeneration-related gene expression during finfold regeneration. (A–H) Whole-mount in situ hybridization analysis. The expression of regeneration-response genes phlda2 (A), mvp (B), and fn1b (C, D), mmp9 (E–G), and (H) was observed after 1 hpa (E), 3 dpa (A–C, F) or 6 hpa (D, G, H). The induction of mmp9 and fn1b was slightly earlier (approximately 50% of larvae exhibited a low level of gene expression at 1 hpa). In contrast to the expression of these genes, that of dlx5a, one of the epithelial markers ([Kawakami et al., 2004] and [Yoshinari et al., 2009]), was never observed at 6 hpa (H). Gene expression was examined by using more than 10 larvae. (I) A summary of temporal gene expression during regeneration. The red color indicates the unique immediate early expression of jun family genes. The scale bar represents 100 μm in (A).
Reprinted from Developmental Biology, 340(2), Ishida, T., Nakajima, T., Kudo, A., and Kawakami, A., Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish, 468-479, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.