ZFIN Image: Iwase et al., 2007, Fig. 3

Image

Figure Caption

Fig. 3 Overexpression of SMCX Resulted in Reduction of H3K4me2/3 Signals In Vivo

Expression construct carrying HA-SMCX (A–I) or a mutant HA-SMCX (H514A, J–R) was transiently transfected into U2OS cells and stained by anti-HA (green) and appropriate antibodies against methylated histones (H3K4me1, H3K4me2, or H3K4me3; red). Nucleus was counterstained by Hoechst 33342. Comparing to untransfected cells, significant decrease of K4me2 and K4me3 level was found in SMCX overexpressing cells (C and F, arrows). The demethylation activity was completely abrogated by H514A mutation (O and R, arrows). Overexpression of neither wild-type SMCX nor SMCX (H514A) affected the K4me1 status (C and L, arrows).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 128(6), Iwase, S., Lan, F., Bayliss, P., de la Torre-Ubieta, L., Huarte, M., Qi, H.H., Whetstine, J.R., Bonni, A., Roberts, T.M., and Shi, Y., The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases, 1077-1088, Copyright (2007) with permission from Elsevier. Full text @ Cell