Image
|
Figure Caption
Fig. 2 Specific Demethylation of Methylated H3K4 on Native Histone by SMCX, SMCY, Plu-1, and RBP2
(A and B) Calf thymus histones (3 μg) were incubated with 3 μg of purified, full-length SMCX, SMCY, RBP2, and PLU-1, respectively, and subjected to western blot analysis using antibodies that specifically recognize methylated histones. Heat-inactivated recombinant proteins were used as controls. Reduced signals were found only for H3K4me3 and H3K4me2 by SMCX and SMCY and for all three methylation states by Plu-1. Nonspecific signals were marked as asterisks.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Reprinted from Cell, 128(6), Iwase, S., Lan, F., Bayliss, P., de la Torre-Ubieta, L., Huarte, M., Qi, H.H., Whetstine, J.R., Bonni, A., Roberts, T.M., and Shi, Y., The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases, 1077-1088, Copyright (2007) with permission from Elsevier. Full text @ Cell