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Fig. 3 Labeling pattern in a strain generated with the Ppv3b-4 construct. (A) In the Ppv3b-4 line, GFP expression occurs in the anterior macula (AM), posterior macula (PM) and lateral-line neuromasts, of which a representative example is circled. Ectopic peridermal expression is also apparent. (B) At 5 dpf, fluorescence is observed in hair cells of infraorbital neuromast 3 (Raible and Kruse, 2000). (C) In a 5-dpf transgenic larva, the L1 neuromast of the posterior lateral line (Ghysen and Dambly-Chaudiere, 2004) shows much stronger GFP expression in hair cells than in the periderm. (D) The anterior macula of a transgenic fish fixed at 2 dpf and labeled with phalloidin (red) shows GFP expression in the presumptive hair cells prior to hair-bundle formation. The weak phalloidin signal may represent microvilli on the apical surfaces of hair cells and supporting cells. (E) Phalloidin labeling (red) of the anterior macula in a 5-dpf larva reveals the presence of hair bundles (HB) on transgene-labeled hair cells. The scale bars represent 5 μm.
Reprinted from Gene expression patterns : GEP, 10(2-3), McDermott, B.M., Asai, Y., Baucom, J.M., Jani, S.D., Castellanos, Y., Gomez, G., McClintock, J.M., and Hudspeth, A.J., Transgenic Labeling of Hair Cells in the Zebrafish Acousticolateralis System, 113-118, Copyright (2010) with permission from Elsevier. Full text @ Gene Expr. Patterns