ZFIN Image: Ko et al., 2010, Fig. 3

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Figure Caption

Fig. 3 Patterning in bromi,Gli3^xt and bromi,Gli2 Double Mutants
(A) FoxA2, Nkx2.2, and HB9 immunostaining of E10.5 WT, bromi, Gli3^xt, and bromi,Gli3^xt double-mutant spinal neural tubes. Expression of these markers was normal in Gli3^xt mutants, but disruption of Gli3 restored floor plate specification in bromi,Gli3^xt double mutants.
(B) FoxA2, Nkx2.2, and HB9 immunostaining of E10.5 WT, bromi, Gli2, and bromi,Gli2 double-mutant spinal neural tubes. Ventral neural patterning is similar in bromi and Gli2 single mutants. bromi,Gli2 double-mutant neural tubes lacked Nkx2.2⁺ cells, whereas they were always detected, albeit in reduced numbers, in either single mutant.

Acknowledgments

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Reprinted from Developmental Cell, 18(2), Ko, H.W., Norman, R.X., Tran, J., Fuller, K.P., Fukuda, M., and Eggenschwiler, J.T., Broad-Minded Links Cell Cycle-Related Kinase to Cilia Assembly and Hedgehog Signal Transduction, 237-247, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell