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Fig. 6 The abnormal rhombomere progression and ventricle expansion in mypt1 mutants is myosin dependent. (A) Schematic of blebbistatin rescue of the mypt1 mutant phenotype. (B) Experimental outline. Zebrafish embryos from an mypt1 heterozygous cross were sorted at 20 hpf based on an early notochord and somite phenotype in mypt1 mutants (J.H.G., unpublished). After DMSO control or blebbistatin treatments, brains were ventricle injected and imaged. Representative images from three independent experiments. (C,D) Dorsal and lateral images of a wild-type embryo treated with DMSO control; all embryos appeared wild type (n=56/56). (E,F) Dorsal and lateral images of a wild-type embryo treated with 50 μM blebbistatin; 45% had a slightly less inflated ventricle (n=36/80), but all still had normal brain morphology. (G,H) mypt1 mutant embryo treated with DMSO; all had the mutant phenotype (n=22/22). (I,J) mypt1 mutant rescued with blebbistatin; 79% of mutants were rescued (n=33/42). Asterisks indicate the ear. Anterior is to the left.