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Fig. S6 Rescue procedure. Relatively old (older than 6 months) tkkrk4/rk4 female fish were crossed with tkkrk4/rk4 male fish to produce mildly affected or non-phenotypic offspring. Rescue plasmid (Fig. 3A, Fig. 5G) and Tol2 transposase RNA were injected into these 1-cell stage embryos. The tkkrk4/rk4 female fish that were positive for the transgenes were raised to adulthood and crossed with tkkrk4/rk4 male fish. GFP/Venus+ (transgenic) and GFP/Venus- (non-transgenic) embryos were sorted after fertilization and their phenotypes were classified at 24 hpf (Fig. 3B, Fig. 5H).
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