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Fig. S4 Suppression of BBS6, BBS10, and BBS12 expression in HEK293T cells. Expression of BBS6, BBS10, and BBS12 was blocked by RNA interference (RNAi) in HEK293T cells, and the efficiency and specificity of RNAi was confirmed by quantitative RT-PCR (A) and immunoblotting (B). For quantitative RT-PCR, relative mRNA levels of endogenous BBS genes were compared after normalization with RPL19. For immunoblotting, myc-tagged expression plasmids were cotransfected with control or corresponding BBS gene siRNAs. Equal amounts of myc-GFP expression plasmids were cotransfected and used to assess transfection efficiencies and off-target effect of RNAi.