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Fig. S3 The neuronal cell death phenotype is p53 dependent. A-F: Acridine orange staining of wild-type embryos (A,B), embryos injected with irxl1 morpholino oligonucleotides (MO; C,D) and embryos injected with both irxl1 MOI and p53 MO (E,F) at 25 hours postfertilization (hpf). G: Semiquantitative reverse transcriptase-polymerase chain reaction analysis of Δ113p53 and p21 expression in wild-type, irxl1 MOI morphant, irxl1 and p53 MO morphant, and control (Con) embryos at 25 hpf. Scale bar = 300 μm in A,C,E, 100 μm in B,D,F.
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