Fig. 8 The arch cartilage patterning is disrupted in irxl1 morphants. Cartilage elements were stained with Alcian blue at 5 dpf. A-E: Lateral views. F-J: Ventral views. A,F: Wild-type. B-D,G-I: Morphants of MOI (0.5 pmol plus 0.75 pmol p53 MO) and MOII (0.5 pmol) exhibiting different degrees of cartilage defects are shown as indicated. E,J: MOII morphants rescued by co-injection of irxl1(a+b) cRNA. Arrows indicate the malformed ch (B,I) and pq (H) cartilages; double arrows (H) indicate an increased angle between the ceratohyal cartilages. bb, basibranchials; cb, ceratobranchials; ch, ceratohyal; hys, hyosymplectic; m, Meckel′s cartilage; pq, palatoquadrate. Scale bar = 100 μm.
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