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Fig. 7 Expression of early neural crest cell markers is affected in irxl1 morphants. A-O: Expression of snail2 of embryos at 18 hours postfertilization (hpf; A-F) and sox9b at 18 hpf (G-I), and 24 hpf (J-O) are examined by whole-mount in situ hybridization. A,D,G,J,K: Wild-type (WT). B,E,H,L,M: irxl1 MOI (plus p53 MO) morphants. C,F, I, N,O: irxl1 MOII morphants. A-C,G-I,J,L,N: Lateral views. D-F,K,M,O: Dorsal views. Arrows indicate reduced expression domains. I-III indicate the three streams of migrating neural crest cells. ov, otic vesicle. Scale bar = 200 μm.
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