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Fig. 6 VE-cadherin knockdown in fli1:GFP embryos reproduces and allows measurement of endocardial/myocardial separation at 32 hpf.
Images taken from movies of transgenic fli:GFP embryos that were injected with either control MO or VE-cad MO and observed with fluorescence microscopy (a,b). These images demonstrate increased endocardial/myocardial separation. The average measured distance between endocardium and myocardium (white arrows) differed significantly between the two groups, with the knockdown embryos consistently having a larger separation between the two layers (c).
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