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Fig. 6 BTS inhibits skeletal muscle contraction and suppresses thick and thin filament assembly in skeletal muscles of zebrafish embryos.
A–D. Morphological comparison of control (A, B) or BTS-treated (C, D) embryos at 48 hpf (A, C) and 120 hpf (B, D). Compared with control (B), BTS-treated embryos (D) showed a clear edema (indicated by the arrow) at 120 hpf. E and F. Anti-MyHC antibody (F59) staining shows the organization of thick filaments in slow muscles of control (E) or BTS-treated (F) embryos at 60 hpf. G and H. Anti-MLC antibody (F310) staining shows the organization of thick filaments in fast muscles of control (G) or BTS-treated (H) embryos at 72 hpf. I and J. Anti-α-actin antibody staining shows the organization of thin filaments in slow muscles of control (I) or BTS-treated (J) embryos at 60 hpf. K and L. Anti-α-actin antibody staining shows the organization of thin filaments in fast muscles of control (K) or BTS-treated (L) embryos at 72 hpf. Scale bars = 100 μm in A and B, 25 μm in E.