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Fig. 2 col27a1a is expressed in notochord and cartilage.
(A–I) col27a1a was expressed in the notochord at early stages (black arrow), and (G) in the adaxial cells of the somites by 13 somite stage (arrowhead). At 24 hpf, expression was also observed in the (I) fin fold (black arrowhead) and in the (K) floor plate (black arrow) and hypochord (black arrowhead), surrounding the notochord. (J) Sense probe control was intentionally overexposed and revealed an absence of notochord staining. (L–P) Beginning at 48 hpf, strong expression of col27a1a was seen in the cartilage elements of the head and continued through 5 dpf, the latest time point analyzed. (M) Expression was also seen in the first forming tooth. Scale bar in K represents 20 μm. Scale bars in remaining images represent 100 μm. Scale bar in A applies to B–H. Scale bar in M applies to N–P. bsr, branchiostegal ray; cb, ceratobranchial arches; ch, ceratohyal; cl, cleithrum; d, dentary; e, ear; ep, ethmoid plate; m, maxilla; op, operculum; s, somites; t, tooth; tb, trabecule.