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Fig. S2 RT-PCR of trypsins in gill, mouth, and nose from zebrafish. Tissues from each organ were obtained using microscissors. RNA from tissues was isolated using Absolutely RNA prep kit from Stratagene, Inc. Trypsin primers were designed using MALDI-TOF data and NCBI database (Forward 5′-TCATGCTGATCAAGCTGA-3′ Reverse 5′-ATCCAGCGCAGAACATG-3′).
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