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Fig. 1 External phenotype, genotype, cloning and morpholino phenocopy of xav mutants.
A. External xav and etfdh morphant (MO) phenotypes at ∼60 hpf include a bent and thinner tail and smaller head and eyes. Scale bar = 100 μm. B. xav mutants and MO exhibit slower heart beat (WT 158±6 beats/minute, N = 13 embryos; xav 87±7; N = 14 embryos; MO 65±7, N = 14 embryos; one-way ANOVA, followed by Dunn′s pairwise comparison, * p<0.001. C. Genetic and physical map of the xav (zgc:92093) locus (red), including microsatellite and SSR markers, number of recombinants, and BAC clones from the T51 radiation hybrid panel. D. etfdh mutation in xav is a T to A mutation (blue box) resulting in a premature stop codon (red box). The amino acid sequence of etfdh is highly conserved among several species, from C. elegans to human. E. Schematic location of xav mutation, resulting in truncation of the C terminal. etfdh MO is predicted to give rise to a protein fragment lacking all functional domains.