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Fig. S3 xav mutants display polycystic kidney like phenotypes. Immunostaining of whole mount zebrafish embryos revealed that the cilia in the pronephric ducts, as labeled by immunostaining with anti-acetylated tubulin antibody, appear distended and irregularly thickened, and contain gaps, in xav mutants compared to WT embryos at ∼60 hpf. Pronephric duct epithelial cells, as labeled by immunostaining with the anti-NaK ATPase antibody α6F, appear irregular in shape and showed aberrant clustering in xav embryos. N = >10 xav and WT embryos for each immunostaining assessment. Scale bar = 20 μm.
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