Fig. S2

Fig. S2 Titration of DEAB and aldh1a tMO. Wild type (A), aldh1a2^um22 mutant (B), aldh1a2ⁱ²⁶ mutant (C), DEAB-treated (D-G) and aldh1a2 tMO-injected (H-K) embryos were assayed for pdx1 expression at 48 hpf. DEAB and aldh1a2 tMO was titrated as indicated (D-G and H-K, respectively). Black arrows indicate pancreas expression and open arrows indicate duodenum expression of pdx1. Note that intermediate concentrations of DEAB (1 μM, panel E) and aldh1a2 tMO (250–500 uM, panels I, J) produce similar phenotypes to the aldh1a2^um22 and aldh1a2ⁱ²⁶ mutants. Embryos are in dorsal view with anterior to the left.