ZFIN Image: Lee et al., 2009, Fig. 1

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Figure Caption

Fig. 1 The spatiotemporal expression of FoxD5 during zebrafish embryogenesis. Using RT-PCR to detect the transcripts of FoxD5 during the developmental stages as indicated. Lane M was a marker, and tubulin was an internal control (A). Using WISH to reveal that FoxD5 was expressed ubiquitously at 6 hpf (B), expressed in the yolk synthetic layer and adaxial cells at 8-hpf (C), and expressed in the anterior PSM, adaxial cells and tailbud at 10- (D) 12- (E) and 14-hpf (F). During 18–24 hpf, FoxD5 in the adaxial cells was down-regulated gradually (black blanket), but it was still expressed in the head and tailbud (G, H). During 12 hpf, dynamic stripe expressions of FoxD5 in the anterior PSM (S0-S-II) and newly formed somites (SI) were observed (I, J). Double ISH of mespa, her1 (labeled in red) and FoxD5 (labeled in blue) expressions during 12 and 14 hpf showed that FoxD5 was expressed in the anterior PSM from S-I to S-II (K, L).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 336(2), Lee, H.C., Tseng, W.A., Lo, F.Y., Liu, T.M., and Tsai, H.J., FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis, 232-245, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.