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Fig. 2 BMP-15 inhibits mPRβ, but not mPRα expression. To overexpress BMP-15, oocytes were microinjected with plasmid DNA carrying the coding sequence of zebrafish BMP-15 (1 ng/follicle) or equal amount of the empty vector as the negative control. To knockdown BMP-15, oocytes were injected with an antisense oligonucleotide (0.5 ng/follicle) targeting the translation initiation site of BMP-15. A missense oligonucleotide was used as the negative control. Proteins were extracted at 18 h after microinjection and expression of the mPRα and mPRβ was detected by Western blot analysis using mPRα and mPRβ antibodies. Equal loading was confirmed by Western blotting probed with an α-tubulin antibody. Data represent mean ± S.E.M. of five experiments.*p < 0.05. EV, empty vector control. aS, antisense oligonucleotides; mS, missense oligonucleotides.
Reprinted from Molecular and Cellular Endocrinology, 312(1-2), Tan, Q., Zagrodny, A., Bernaudo, S., and Peng, C., Regulation of membrane progestin receptors in the zebrafish ovary by gonadotropin, activin, TGF-beta and BMP-15, 72-79, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.