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Fig. 6 β-Arrestin1 Relieves PcG Repression and Regulates Hematopoietic Lineage Specification
(A–D) Whole-embryo chromatin immunoprecipitation (E-ChIP) assays at 12 hpf. Embryos injected with 4 ng control morpholino (Ctrl MO), 4 ng β-arrestin1 morpholino (Arrb1 MO), 4 ng β-arrestin1 morpholino plus 100 pg human β-arresetin1 mRNA (hArrb1 mRNA) or 4 ng β-arrestin1 morpholino plus 100 pg human 7M β-arrestin1 mRNA (hArrb1 7M mRNA) were probed with antibodies against YY1 (A), Suz12 (B), H3K27me3 (C) and H3K4me3 (D). The presence of cdx4, hoxa9a, hoxb4a and cdx1a promoter sequences in the input DNA and antibody-bound chromatin segments was analyzed by qPCR. The data obtained were normalized on the basis of the corresponding input control, and means ± SEM from three independent experiments were plotted, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control morpholino group. Primer sets covering different regions of the same promoter were used and produced similar results.
(E) Phenotypic analyses of embryos at 26 hpf. Embryos injected as (A)–(D) were analyzed.
(F–N) Whole-mount in situ hybridization for embryos at 12 hpf. Embryos injected as in (E) were hybridized with riboprobes to cdx4 (F–H), gata1 (I–K) and scl (L–N).
Reprinted from Cell, 139(3), Yue, R., Kang, J., Zhao, C., Hu, W., Tang, Y., Liu, X., and Pei, G., Beta-arrestin1 regulates zebrafish hematopoiesis through binding to YY1 and relieving polycomb group repression, 535-546, Copyright (2009) with permission from Elsevier. Full text @ Cell