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Fig. 6 TGF-β1 inhibits mPRβ expression in a dose-dependent manner. Mid- to late growth phase follicles were treated with Cortland′s medium, or TGF-β1 at 0.1, 1, or 10 ng/ml for 18 h. Proteins were extracted and expression of mPRα and mPRβ was detected by Western blot analysis using anti-mPRα and mPRβ antibodies. Equal loading was confirmed by Western blotting probed with an anti-α-tubulin antibody. Histograms show normalized densitometry data (mean ± S.E.M.) from three experiments. *p < 0.05 vs. control.
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Reprinted from Molecular and Cellular Endocrinology, 312(1-2), Tan, Q., Zagrodny, A., Bernaudo, S., and Peng, C., Regulation of membrane progestin receptors in the zebrafish ovary by gonadotropin, activin, TGF-beta and BMP-15, 72-79, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.