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Fig. 5 Activation of Wnt signaling rescues loss of dorsal eye markers in Dkk1-expressing embryos. (A-H) Dkk1-expressing embryos were treated with the Wnt pathway activator LiCl (150 mM) from 11-14 hpf, heat shocked at 12 hpf, and fixed at 24 hpf. The expression of tbx5 (A-D) and gdf6a (E-H) were analyzed by in situ hybridization. LiCl led to an expansion of tbx5 and gdf6a expression in embryos not expressing Dkk1 (B,F), and a rescue of tbx5 and gdf6a in embryos expressing Dkk1 (D,H). Lateral views, dorsal up, anterior left.
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