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Fig. S1 Formation of complete secondary embryonic axes. (A-D) Formation of a complete secondary axis, extending anteriorly up to the anterior head and containing axial tissue, after injection of noggin1 and frzb RNAs into a ventral blastomere at the 16-cell stage in lateral (A) and frontal (B) views. (C) Detail of the trunk region in lateral view. An identical secondary axis can be obtained upon overexpression of nodal by injection of ndr2 RNA into a ventral blastomere at the 16-cell stage, as shown in lateral view (D).
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