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Fig. 4 prdm1a is necessary for proliferation within the arches. A: A 48-hpf control embryo stained with anti-phosphohistone H3. B: As compared to control, a 48-hpf prdm1a mutant embryo has a reduction in proliferation in the arch region. C: Average cell proliferation at 24, 36, and 48 hpf in control and prdm1a mutant embryos. Boxed area indicates approximate region in which cell counts were measured and analyzed using Image J and found to not be different in size (not shown). Number of embryos examined for 24 hpf, n = 17; 36 hpf, n = 27; and 48 hpf, n = 13.
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