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Fig. 4
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Fig. 4 Interaction with Taf3 is required for Trf3 function in zebrafish development. A: Whole-mount in situ hybridization with a riboprobe to hbae1 (22 hpf) in trf3 MO-treated embryos injected with mRNAs expressing tbp, trf3, or a trf3 mutant. Arrowheads denote staining in the intermediate cell mass (ICM). B: Whole-mount in situ hybridization with riboprobes to gata1 or pax2 (14 hpf). C: RT-PCR analysis of scl1, lmo2, or, as a control, gapdh expression at 18 hpf.
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