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Fig. S2 In order to count β-cells in the principal islet, we used the tg(ins:hmgb1-eGFP) transgenic line that expresses eGFP in the nuclei only. This allows the visualization of individual nuclei surrounded by a negative cytoplasm. The whole principal islet is between 45–50 μM thick and we imaged 2 μM Z-sections through the islet. In this way each nuclei is captured on multiple sections. Each section was magnified and projected onto paper. By outlining each nucleus, a detailed record of the number of GFP positive nuclei could be recorded.
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Reprinted from Mechanisms of Development, 126(10), Parsons, M.J., Pisharath, H., Yusuff, S., Moore, J.C., Siekmann, A.F., Lawson, N., and Leach, S.D., Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas, 898-912, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.