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Fig. S2 Whole mount in situ hybridization showing n-cor mRNA is widely present in zebrafish during early development. (A–E) animal pole top; (F–H) anterior left; (A–G) lateral view; (H) flat mount and dorsal view. A, 16-cell stage (1.5 hpf:); B, 512–cell stage (2.75 hpf); C, 30% epiboly (4.7 hpf); D, 50% epiboly (5.3 hpf); E, bud stage (10 hpf); F, 18-somite stage (18 hpf); G and H, 24 hpf. N-CoR mRNA message is widely distributed in early developmental stages from cleavage stage through gastrulation stage (A–D). At bud stage, the expression of n-cor is richer in anterior part than in posterior part (E). From 18 hpf to 24 hpf, n-cor message is mainly expressed in head region with very low expression in the other parts (F–H). Flat mount of 24 hpf embryos shows the expression is mainly in ventricles of forebrain, midbrain and hindbrain, and also in retina (H).
Reprinted from Mechanisms of Development, 126(10), Xu, F., Li, K., Tian, M., Hu, P., Song, W., Chen, J., Gao, X., and Zhao, Q., N-CoR is required for patterning the anterior-posterior axis of zebrafish hindbrain by actively repressing retinoid signaling, 771-780, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.