ZFIN Image: Ebarasi et al., 2009, Fig. S2

Image

Figure Caption

Fig. S2 Tight junctions persist in crb2b MO. (A) Electron micrographs of podocytes from control 4 dpf larvae shows the cell bodies, primary processes (asterisk), and the regular patterned array of foot processes along the GBM; GBM (black arrows). (B, C) In crb2b morphants, podocytes are attached to a GBM (black arrow in C), but the organization of regularly spaced foot processes is lost. Slit diaphragms are absent. Ectopic tight junctions are visible in morphant podocytes (white arrows in B and C). In addition, blunt ended apical membrane projections are found extending into the Bowman′s space (black arrowheads in B and C). A, B 20,000x magnification; C 40,000x magnification.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 334(1), Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A., A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier, 1-9, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.