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Fig. S1 crb2b expression in the pronephric glomerulus. (A, B) Wholemount in situ hybridization reveals crb2b expression in the glomerular podocytes and retina at 4 dpf and 2 dpf (G). Arrows point to the podocyte expression. (C, D, E) DIC images of transverse sections through a 4 dpf larval glomerulus stained with the crb2b antisense in situ probe. Notice that crb2b expression is not observed in the tubular epithelia (arrowheads; n, notochord). (F) Transverse section through a 4 dpf glomerulus double stained with α-panCrb (green) and α-ZO-1 (red) antibodies. Nuclei are stained with DAPI.
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Reprinted from Developmental Biology, 334(1), Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A., A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier, 1-9, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.