ZFIN Image: Ebarasi et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Phenotype of the crb2b morphants. (A) Control mismatch and (B) crb2b-ATG injected 2.5 dpf larvae. Note the pericardial edema (arrowhead) and pronephric cysts (arrow). (C, D) Histological sections at the level of the glomerulus in control (C) and crb2b-ATG MO (D). Note the expanded Bowman′s space (asterisk) in D. (E, F) Staining with ±-Na⁺/K⁺ ATPase in the pronephric tubules and ducts is not generally affected in crb2b MO. (G–L) In situ hybridization on 2 dpf wildtype (G, I, K) or crb2b-ATG (H, J, L) with wt1 (G, H), podocin (I, J), or nephrin (K, L) antisense probes. Cells expressing wt1, nephrin, and podocin are present in crb2b morphants (black arrowheads).

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A., A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier, 1-9, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.