Fig. S3

Fig. S3 Classification of pcdh19 morphants. Shown are 1 day old zebrafish embryos that are uninjected (WT), or that show increasing severity of morphant phenotype (MILD, MODERATE, SEVERE). Mild embryos are those that exhibit little or no abnormalities of the trunk or the eyes, have a roughly normal-sized brain, but exhibit minor irregularities in the midbrain–hindbrain region. Moderate embryos tend to have slightly smaller eyes, shorter brains along the antero-posterior axis and have a much more disorganized midbrain–hindbrain area. Severe morphants have curved or shortened trunks, severely disorganized brains, ectopic cell masses in the ventricles and, occasionally, areas of pyknotic nuclei. These classes were identical for each of the morpholinos tested (Mo-T, Mo-S1 and Mo-S2), and served as a reliable basis for scoring morpholino injections.