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Figure Caption
Fig. S5 (A) Rabbit polyclonal antibodies directed against zebrafish Nephrin specifically recognize a single approximately protein in agreement with its predicted 140 ka size. Cell lysates from pcDNA3.1 (vector only, P) and zebrafish nephrin transfected HEK-293 cells, N. (B) Immuno-electron micrograph on wildtype podocytes with α-Nephrin antibody. Gold particles are found on the inner aspect of the slit diaphragms (arrowheads).
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Reprinted from Developmental Biology, 334(1), Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A., A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier, 1-9, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.