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Fig. S3 Difference in sarcolemmal pathology between sof and caf. (A-C) A direct comparison between caf and sof mutant embryos confirms that detached fibres in caf remain impermeable to Evans Blue, with the dye leaching into the space created by adjacent retracting fibres (arrow in B). (D-F) By contrast, Evans Blue readily enters a retracted fibre in a sof embryo, with the detached membrane displaying a faceted appearance. (A,D) DIC images; (B,E) fluorescence images; (C,F) merge of DIC and fluorescence images. Dotted lines in all panels demarcate the outline of detached fibres; all panels show lateral views with anterior to the left.