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Figure Caption
Fig. S12 ZnSR100 pre-mRNA splicing is blocked in embryos treated with splice blocking morpholino oligonucleotide (MOspl). ZnSR100 transcript splicing patterns in the vicinity of exon 5 and intron 5 were analyzed in WT and nSR100 MOspl morphant embryos at 50hpf. A near complete disappearance of fully spliced transcripts along with the accumulation of transcripts with retained intron 5 could be observed in morphant embryos relative to WT embryos.
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Reprinted from Cell, 138(5), Calarco, J.A., Superina, S., O'Hanlon, D., Gabut, M., Raj, B., Pan, Q., Skalska, U., Clarke, L., Gelinas, D., van der Kooy, D., Zhen, M., Ciruna, B., and Blencowe, B.J., Regulation of vertebrate nervous system alternative splicing and development by an SR-related protein, 898-910, Copyright (2009) with permission from Elsevier. Full text @ Cell