Fig. 2

Fig. 2 Expression of cxcr4a, cxcr7b and sdf1b in the developing pharyngeal arches. Lateral views, anterior to the left. Single channel (A, B, D, E, G, H, J, K, M, N, P, Q, S, T) and merged (C, F, I, L, O, R, U) images of confocal micrographs of double fluorescent in situ hybridization of cxcr4a, cxcr7, and sdf1b along with tissue specific marker expression in the pharyngeal arch region. Neural crest markers are indicated in green with cxcr4a (red) in A–O, and endodermal marker nkx2.3 (red) with sdf1b or cxcr7b (green) in P–U. (A–C) CNCC marker dlx2a expression (green) overlaps cxcr4a (red) in a subset of CNCCs (yellow, arrows) at 25 hpf. (D–F) barx1 is coexpressed in a subset of cxcr4a cells at 25 hpf. (G–I) At 28 hpf, hand2 is expressed in the ventral-most domain of arch CNCCs and partially overlaps with cxcr4a in this domain (yellow, arrows). (J–L) cxcr4a (red) is mostly excluded from sox10 nonectomesenchymal expression at 19 hpf (green). (M–O) The pan CNCC marker crestin is expressed in a broad domain in the pharyngeal arch region, where cxcr4a is also expressed. cxcr4a is excluded from the dorsal crestin domain that corresponds to nonectomesenchymal NCCs. (P–R) sdf1b (green) and the endodermal marker nkx2.3 (red) overlap in pouch 2 (arrows) at 28 hpf. (S–U) cxcr7b (green) is coexpressed with nkx2.3 (red) throughout pharyngeal arch endoderm at 31 hpf (yellow; arrowheads).