|
Fig. 3b (E) Coronal section through telencephalon showing double-label of fezf2-GFP and proliferation marker PCNA (20x magnification). (E′–E″′) Single confocal Z-section of the boxed region shows that some fezf2-GFP+ cells colocalize with PCNA (arrowheads) (40x magnification). (F) Coronal section through telencephalon showing double-label of fezf2-GFP and BrdU (Bromodeoxyuridine, S-phase marker) (20x magnification). (F′–F″′) Single confocal Z-section of the boxed region shows that some fezf2-GFP+ cells colocalize with BrdU (arrowheads) (40x magnification). (G) Coronal section through telencephalon showing double-label of fezf2-GFP and PH3 (phospho-histone H3; marker of mitosis) (20x magnification). (G′–G″′) Single confocal Z-section of the boxed region shows colocalization of a fezf2-GFP+ cell with PH3 (arrowhead) (100x magnification). (H) Coronal section through telencephalon showing colocalization of fezf2-GFP with Hu (neuronal marker) in the subpallium (Vl region) (20x magnification). (H′–H″′) Single confocal Z-section of the boxed region shows colocalization of fezf2-GFP with Hu in the Vl region (40x magnification). Abbreviations: D, dorsal telencephalon; V, ventral telencephalon; VZ, ventricular zone; OB, olfactory bulb; Vd, dorsal nucleus of V; Vl, lateral nucleus of V; Vv, ventral nucleus of V.
Reprinted from Gene expression patterns : GEP, 9(6), Berberoglu, M.A., Dong, Z., Mueller, T., and Guo, S., fezf2 expression delineates cells with proliferative potential and expressing markers of neural stem cells in the adult zebrafish brain, 411-422, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns