ZFIN Image: Feijoo et al., 2009, Fig. 6

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Figure Caption

Fig. 6 Shh, Six3.1, and Axud1 interactions. A-D: Wild type embryos. E-H: axud1 morphant embryos. A, E: Acridine orange stain of control (A) and experimental embryos (E). Cell death is detected throughout the cephalic region. B, F: Proliferation analysis evidenced by the anti-phospho-histone H3 (H3-P) immunostain in wild type (B) and axud1 morphants (F). Note the reduced number of H3-P-labelled cells along the bracket in morphant embryos compared with the wild type siblings. C, G: axud1 in situ hybridization in control (C) and in smu^b577 mutant embryos (G). An evident decrease in axud1 expression is detected in embryos with reduced levels of Shh pathway activity. Inset in G shows that in situ stain has labeled the notochord but not the head region. D, H: six3.1 in situ hybridization in wild type (D) and axud1 morphant embryos (I). Strong inhibition of six3.1 transcription is observed in experimental embryos. MB-HB, midbrain-hindbrain boundary; e, eye field; t, telencephalon; tc, tectum. All panels are lateral views of 24-hpf embryos.

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Acknowledgments

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